purjfication of high molecular weight cellulolytic enzymes from celluwmonas sp. strain 0

purification of extra-cellular cellulolytic enzymes from c e l l u l o mssp. strain 0, isolated from forest soil in the north of iran , was studied by using gel filtration and ion-exchange chromatography. two endo-glucanases (endo-i & endo-ii) and one exo-glucanase (exo-i) were purified to hornogenity . the purified enzymes had molecular weights of about 39.000,70.000 and 90.000 daltons for endo-i, endo-ii and exo-i, respectively. heavy metal ions, like fe and cu inactivated the enzymes by about 80%, while zn and co had no effect on the enzyme activities